Administrators acting for HMV have today confirmed that the Hemel Hempstead store is in line for closure.
The Riverside Shopping Centre branch was initially saved from the axe in the first wave of closures but has been listed among a further 37 stores that will shut their doors.
The latest decision affects a total of 464 staff.
No date has been set for the closures but they are expected to take place over the next four to six weeks.
Joint administrator Nick Edwards said: “As part of our ongoing review of HMV’s financial position, we have undertaken a further review of the store portfolio and have identified an additional 37 stores for closure. This step has been taken in order to enhance the prospects of the restructured business and continuing as a going concern.
“Together with the previously identified 66 closures, this restructuring will result in a residual portfolio of some 116 stores.